|Flow Cytometry (Flow)||Assay Dependent|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||C57BL/10 splenic T cells and concanavalin A stimulated C57BL/10 splenocytes|
|Excitation/Emission Profile||View spectra|
|Storage buffer||PBS with sucrose, 4mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C|
The M1/70.15 monoclonal antibody (mAb) reacts with the alpha-M subunit of Mac-1 (CD11b/CD18). For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm). Applications for the M1/70.15 mAb include immunoprecipitation, immunostaining for flow cytometry, IHC of acetone-fixed frozen sections, and blocking of CR3-mediated rosetting.
Use dim light during handling, incubation with cells and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Every lot is tested by flow cytometry using freshly harvested mouse blood.
CD11b is implicated in various adhesive interactions of monocytes, macrophages, and granulocytes as well as in mediating the uptake of complement-coated particles. alpha-M subunit of Mac-1 (CD11b/CD18) is a type three complement receptor (CR3), the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, Factor 5X, and ICAM-1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, and blood monocytes (B-1 cells), and plays a role in leukocyte endothelial interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD11 antigen-like family member B; CD11B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; CR3A; Integrin alpha M; integrin alpha-M; leukocyte adhesion receptor MO1; MAC-1; Mac-1 alpha; MAC1A; macrophage antigen alpha; MGC117044; MO1A; SLEB6
Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1
Entrez Gene ID: (Mouse) 16409
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