Flow cytometry analysis of CD11b was performed on untreated (blue line) or ATRA treated (red line) HL-60 cells. After 72 hours incubation with or without 10 µM ATRA, cells were fixed and washed with PBS. Fixed HL-60 cells were incubated with 1 µg of CD11b monoclonal antibody (Product # MA1-19004), followed by incubation with a DyLight 488-conjugated goat-anti-mouse IgG secondary antibody (Product # 35502). To control for non-specific binding, cells were also incubated with secondary antibody only (black line). Incubations were performed for 30 mins in the dark. 10,000 cells at were analyzed for each sample.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages.
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.