|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat peritoneal macrophages|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100 (10 µl for 10^6 cells in 100 µl)|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunoprecipitation (IP)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-90756 detects CD11b from rat samples.
MA1-90756 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation applications.
The MA1-90756 immunogen is: Rat peritoneal macrophages
CD11b, also known as integrin alpha-M (ITGAM) or integrin alpha-X (ITGAX), is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These adhesions are critical for cell-cell and cell-matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mononuclear phagocyte xanthine oxidoreductase contributes to cytokine-induced acute lung injury.
MA1-90756 was used in flow cytometry to determine the role of xanthine oxidoreductase to lung injury.
|Wright RM,Ginger LA,Kosila N,Elkins ND,Essary B,McManaman JL,Repine JE||American journal of respiratory cell and molecular biology (30:479)||2004|