|Tested species reactivity||Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat peritoneal macrophages.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:100|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 12 publications below|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
mouse homolog is an integrin alpha subunit that interacts with integrin beta 2 subunit to form Mac-1; involved in immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fluoxetine prevents oligodendrocyte cell death by inhibiting microglia activation after spinal cord injury.
MA1-80774 was used in immunohistochemistry - frozen section to demonstrate that fluoxetine alleviates oligodendrocyte cell death after spinal cord injury
|Lee JY,Kang SR,Yune TY||Journal of neurotrauma (32:633)||2015|
|Not Applicable||Not Cited||
Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis.
MA1-80774 was used in flow cytometry to compare the homing and therapeutic action of cryopreserved subcutaneous adipose tissue and bone marrow-derived mesenchymal stromal cells in a rat model of colitis
|Castelo-Branco MT,Soares ID,Lopes DV,Buongusto F,Martinusso CA,do Rosario A,Souza SA,Gutfilen B,Fonseca LM,Elia C,Madi K,Schanaider A,Rossi MI,Souza HS||PloS one (7:null)||2012|
|Not Applicable||Not Cited||
Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.
MA1-80774 was used in flow cytometry and immunoprecipitation to characterize expression of rat CD11/CD18
|Tamatani T,Kotani M,Miyasaka M||European journal of immunology (21:627)||1991|
A rat model of mild cognitive impairment associated with vascular factor.
MA1-80774 was used in immunohistochemistry - free floating to develop and characterize a rat model of vascular mild cognitive impairment
|Zhang L,Wei WS,Li YJ,Wang Y||Neuropathology : official journal of the Japanese Society of Neuropathology (31:112)||2011|
The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.
MA1-80774 was used in immunocytochemistry to study the optimal siRNA concentration for gene silencing in both astrocytes and microglial cells.
|Ki KH,Park DY,Lee SH,Kim NY,Choi BM,Noh GJ||Korean journal of anesthesiology (59:403)||2010|
The parkinsonian neurotoxin rotenone activates calpain and caspase-3 leading to motoneuron degeneration in spinal cord of Lewis rats.
MA1-80774 was used in immunohistochemistry to use the rotenone-induced model of Parkinsonism to explore spinal cord degeneration
|Samantaray S,Knaryan VH,Guyton MK,Matzelle DD,Ray SK,Banik NL||Neuroscience (146:741)||2007|
Spinal p38beta isoform mediates tissue injury-induced hyperalgesia and spinal sensitization.
MA1-80774 was used in immunohistochemistry (frozen) to characterize two p38 isoforms found in the spinal cord.
|Svensson CI,Fitzsimmons B,Azizi S,Powell HC,Hua XY,Yaksh TL||Journal of neurochemistry (92:1508)||2005|
Activation of p38 mitogen-activated protein kinase in spinal microglia is a critical link in inflammation-induced spinal pain processing.
MA1-80774 was used in immunohistochemistry (frozen) to study the role of p38 mitogen-activated protein kinase in nociception.
|Svensson CI,Marsala M,Westerlund A,Calcutt NA,Campana WM,Freshwater JD,Catalano R,Feng Y,Protter AA,Scott B,Yaksh TL||Journal of neurochemistry (86:1534)||2003|
Spinal p38 MAP kinase is necessary for NMDA-induced spinal PGE(2) release and thermal hyperalgesia.
MA1-80774 was used in immunohistochemistry to test if activation of spinal NMDA-receptor initiates activation of the p38 mitogen-activated protein kinase pathway and spinal release of prostaglandins and hyperalgesia.
|Svensson CI,Hua XY,Protter AA,Powell HC,Yaksh TL||Neuroreport (14:1153)||2003|
LSF-1 may modulate the indirect allorecognition pathway to delay allograft rejection.
MA1-80774 was used in flow cytometry to examine the role LSF-1 in liver regeneration after insult or injury.
|Vari F,Lord R,Goto S||Transplant immunology (10:259)||2002|
Propofol depressed neutrophil hydrogen peroxide production more than midazolam, whereas adhesion molecule expression was minimally affected by both anesthetics in rats with abdominal sepsis.
MA1-80774 was used in flow cytometry to examine the effect of anesthetic treatment on neutrophil function during sepsis.
|Inada T,Taniuchi S,Shingu K,Kobayashi Y,Fujisawa J,Nakao S||Anesthesia and analgesia (92:437)||2001|
Combined TUNEL and double immunofluorescent labeling for detection of apoptotic mononuclear phagocytes in autoimmune demyelinating disease.
MA1-80774 was used in immunocytochemistry to use TUNEL and an immunofluorescent labeling technique to determine the fate of cells in the central nervous system of mice with experimental allergic encephalomyelitis.
|Ray SK,Schaecher KE,Shields DC,Hogan EL,Banik NL||Brain research. Brain research protocols (5:305)||2000|
Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation.
MA1-80774 was used in flow cytometry to discuss tolerance to ACI heart allografts using rats.
|Lan F,Hayamizu K,Strober S||Transplantation (69:649)||2000|
In vivo reversal of amyloid-beta lesions in rat brain.
MA1-80774 was used in immunohistochemistry to investigate the effect of beta-sheet breaker treatment on disassembly of fibrillar amyloid deposits.
|Sigurdsson EM,Permanne B,Soto C,Wisniewski T,Frangione B||Journal of neuropathology and experimental neurology (59:11)||2000|
Comparison of chimeric acid and non-chimeric tolerance using posttransplant total lymphoid irradiation: cytokine expression and chronic rejection.
MA1-80774 was used in flow cytometry to study tolerance induction using heart allografts in Lewis rat.
|Hayamizu K,Lan F,Huie P,Sibley RK,Strober S||Transplantation (68:1036)||1999|
Monocyte-derived dendritic cell precursors facilitate tolerance to heart allografts after total lymphoid irradiation.
MA1-80774 was used in flow cytometry to discuss factors that promote tolerance in a rat ACI heart allografts model.
|Hayamizu K,Huie P,Sibley RK,Strober S||Transplantation (66:1285)||1998|
Donor blood monocytes but not T or B cells facilitate long-term allograft survival after total lymphoid irradiation.
MA1-80774 was used in flow cytometry to determine the subset(s) of cells in donor blood that promote long-term allograft survival in rats.
|Hayamizu K,Zeng D,Huie P,Garcia-Ojeda ME,Bloch DA,Fong L,Engleman EG,Sibley RK,Strober S||Transplantation (66:585)||1998|
Priming in the brain, an immunologically privileged organ, elicits anti-tumor immunity.
MA1-80774 was used in immunohistochemistry (frozen) to report successful immunization against a central-nervous-system tumor.
|Fathallah-Shaykh HM,Gao W,Cho M,Herrera MA||International journal of cancer (75:266)||1998|