|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat peritoneal macrophages.|
|Storage buffer||PBS, pH 7.4, with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD11b, also known as the alpha M integrin chain or MAC-1, is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.
MA1-80775 was used in immunocytochemistry to study the optimal siRNA concentration for gene silencing in both astrocytes and microglial cells.
|Ki KH,Park DY,Lee SH,Kim NY,Choi BM,Noh GJ||Korean journal of anesthesiology (59:403)||2010|