|Tested species reactivity||Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25-1:200 (10 µl for 10^6 cells in 100 µl)|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21591 detects CD11b/c in rat samples.
MA1-21591 has been successfully used in ELISA, FACS and immunohistochemistry (paraffin) procedures.
The MA1-21591 immunogen is rat macrophages.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
CD11b, also known as integrin alpha-M (ITGAM) or integrin alpha-X (ITGAX), is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These adhesions are critical for cell-cell and cell-matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.
MA1-21591 was used in immunocytochemistry to study the optimal siRNA concentration for gene silencing in both astrocytes and microglial cells.
|Ki KH,Park DY,Lee SH,Kim NY,Choi BM,Noh GJ||Korean journal of anesthesiology (59:403)||2010|
Insulin increases resistance to burn wound infection-associated sepsis.
MA1-21591 was used in flow cytometry to investigate the therapeutic efficacy of insulin on the treatment of sepsis
|Gauglitz GG,Toliver-Kinsky TE,Williams FN,Song J,Cui W,Herndon DN,Jeschke MG||Critical care medicine (38:202)||2010|
The parkinsonian neurotoxin rotenone activates calpain and caspase-3 leading to motoneuron degeneration in spinal cord of Lewis rats.
MA1-21591 was used in immunohistochemistry to use the rotenone-induced model of Parkinsonism to explore spinal cord degeneration
|Samantaray S,Knaryan VH,Guyton MK,Matzelle DD,Ray SK,Banik NL||Neuroscience (146:741)||2007|
Priming in the brain, an immunologically privileged organ, elicits anti-tumor immunity.
MA1-21591 was used in immunohistochemistry (frozen) to report successful immunization against a central-nervous-system tumor.
|Fathallah-Shaykh HM,Gao W,Cho M,Herrera MA||International journal of cancer (75:266)||1998|
|Not Applicable||Not Cited||
Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.
MA1-21591 was used in flow cytometry and immunoprecipitation to characterize expression of rat CD11/CD18
|Tamatani T,Kotani M,Miyasaka M||European journal of immunology (21:627)||1991|