|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:1-1:20 (10 µl for 10^6 cells in 100 µl)|
|Immunohistochemistry (Frozen) (IHC (F))||Neat-1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||Neat-1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-21592 detects CD11b/c (FITC) in Rat samples.
MA1-21592 has been successfully used in FACS and Immunohistochemistry (Paraffin) procedures.
MA1-21592 immunogen corresponds to rat macrophages.
For extended storage, store at -20°C, avoiding freeze/thaw cycles.
CD11b, also known as integrin alpha-M (ITGAM) or integrin alpha-X (ITGAX), is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. These adhesions are critical for cell-cell and cell-matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.
MA1-21592 was used in immunocytochemistry to study the optimal siRNA concentration for gene silencing in both astrocytes and microglial cells.
|Ki KH,Park DY,Lee SH,Kim NY,Choi BM,Noh GJ||Korean journal of anesthesiology (59:403)||2010|