|Tested species reactivity||Mouse, Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal region of the mouse CD11 (b/c) protein within residues 500-600.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 4 publications below|
Prior to immunostaining paraffin tissues, antigen retrieval with citrate buffer (pH 6.0) is recommended.
Suggested positive control: Raw 264.7 cell lysate.
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Type III TGF-? receptor downregulation generates an immunotolerant tumor microenvironment.
PA1-46162 was used in immunohistochemistry and western blot to study the generation of an immunotolerant tumor microenvironment in response to downregulation of the Type III TGF-beta receptor
|Hanks BA,Holtzhausen A,Evans KS,Jamieson R,Gimpel P,Campbell OM,Hector-Greene M,Sun L,Tewari A,George A,Starr M,Nixon A,Augustine C,Beasley G,Tyler DS,Osada T,Morse MA,Ling L,Lyerly HK,Blobe GC||The Journal of clinical investigation (123:3925)||2013|
Inflammatory Signals shift from adipose to liver during high fat feeding and influence the development of steatohepatitis in mice.
PA1-46162 was used in immunohistochemistry to investigate the effect of high fat diet on the inflammation in murine adipose tissue and liver
|Stanton MC,Chen SC,Jackson JV,Rojas-Triana A,Kinsley D,Cui L,Fine JS,Greenfeder S,Bober LA,Jenh CH||Journal of inflammation (London, England) (8:null)||2011|
Cell delivery of Met docking site peptides inhibit angiogenesis and vascular tumor growth.
PA1-46162 was used in immunohistochemistry to study the inhibitory effect of Met docking site peptides on angiogenesis and vascular tumor growth
|Cantelmo AR,Cammarota R,Noonan DM,Focaccetti C,Comoglio PM,Prat M,Albini A||Oncogene (29:5286)||2010|
Inducible nitric oxide synthase is present in motor neuron mitochondria and Schwann cells and contributes to disease mechanisms in ALS mice.
PA1-46162 was used in immunohistochemistry to investigate the distribution and function of inducible nitric oxide synthase in motor neuron mitochondria and Schwann cells
|Chen K,Northington FJ,Martin LJ||Brain structure and function (214:219)||2010|