Western blot analysis of Id2 was performed by loading 10ug of Id2-transfected SK-N-BE human neuroblastoma cells per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked for 1 hour. The membrane was probed with an Id2 monoclonal antibody (Product # MA5-14777) at a dilution of 1:500 overnight at 4°C, washed in 0.1% PBS-Tween, and probed with a infrared dye-conjugated anti-rabbit IgG secondary antibody (shown in green) at a dilution of 1:15,000 for 1 hour. To determine equal loading, the blot was also probed with a GAPDH monoclonal antibody (MA5-16757) at a dilution of 1:2000 followed by detection with an infrared dye-conjugate secondary antibody (shown in red) at a dilution of 1:20,000. Detection was performed using a fluorescent scanner. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Asn97 of human Id2 protein|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene.
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