Invitrogen

IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Red 710, eBioscience™

Product Image Gallery

IgD Antibody in Flow Cytometry (Flow) — C57BL/6 mouse splenocytes were unstained (left) or stained with 0.2 µg of IgD Monoclonal Antibody, NovaFluor Red 710 (Product # m030t02r04) (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.

Product Details

M030T02R04

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.2 µg/test	-

Product Specifications
Species Reactivity	Mouse
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	11-26c (11-26)
Conjugate	NovaFluor™ Red 710 Alexa Fluor 700 APC APC-eFluor 780 Biotin eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 660-120S NovaFluor Blue 690 NovaFluor Yellow 590 NovaFluor Yellow 610 PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2926188

Product Specific Information

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.

Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells. Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 639 nm; Emission: 710 nm; Laser: 633-640 nm (Red) Laser

Target Information

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.