Western blot analysis of Influenza A H1N1 HA using A) MDCK cell lysates infected with 4 HAU/ml of A/duck/Alberta/35/1976-like, H1N1 (B) MDCK cell lysates infected with 4 HAU/ml of A/duck/Ukraine/1963-like, H3N8 (C) MDCK cell lysates infected with 4 HAU/ml of A/mallard/Miyagi/53/1976-like, H5N3 (D) MDCK cell lysates infected with 4 HAU/ml of A/duck/Taiwan/33/1993-like, H7N7 (E) MDCK cell lysates infected with 4 HAU/ml of A/turkey/Wisconsin/1/1966-like, H9N2 (F) MDCK cell lysates infected with 4 HAU/ml of A/PR/8/34, H1N1 (G) MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/126/2009, pdmH1N1 and H) MDCK cell lysates infected with 4 HAU/ml of A/Taiwan/3446/2002, H3N2. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Influenza A H1N1 HA polyclonal antibody (Product # PA5-34919) at a dilution of 1:1000.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to Internal region of Influenza A virus HA (A/duck/Alberta/35/1976(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34919 targets Influenza A Virus H1N1 Hemagglutinin (HA) in WB applications and shows reactivity with Virus samples.
The PA5-34919 immunogen is recombinant fragment corresponding to Internal region of Influenza A virus HA (A/duck/Alberta/35/1976(H1N1)).
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.
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