Western blot analysis of Influenza A H1N1 NA using A) 5 ug DF-1 whole cell lysate and B) 5 ug whole cell lysate WSN virus infected DF-1 cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Influenza A H1N1 NA polyclonal antibody (Product # PA5-32238) at a dilution of 1:1000.
|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of Influenza A virus NA (A/WSN/1933(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-32238 targets Influenza A Virus H1N1 NA in WB applications and shows reactivity with Virus samples.
The PA5-32238 immunogen is recombinant protein fragment of Influenza A virus NA (A/WSN/1933(H1N1)).
Influenza A subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. There are 16 different HA antigens (H1-H16) and nine different NA antigens (N1-N9) for Influenza A. The extent of infection into host organisms is determined by HA, which interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cathepsin W Is Required for Escape of Influenza A Virus from Late Endosomes.
PA5-32238 was used in western blot to elucidate how human cathepsin W contributes to influenza A virus replication.
|Edinger TO,Pohl MO,Yángüez E,Stertz S||mBio (6:null)||2015|