|Tested species reactivity||Virus|
|Published species reactivity||Virus, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||M2 protein from A/WSN/33-infected CV1 cell lysate.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Immunoprecipitation (IP)||10 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-082 detects the N-terminal of the influenza A virus M2 protein.
MA1-082 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~15 kDa protein representing the M2 protein. MA1-082 has demonstrated the ability to immunoprecipitate M2 from [35s] cysteine-labeled WSN-infected cells.
MA1-082 has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain.
The MA1-082 immunogen is M2 protein from A/WSN/33-infected CV1 cell lysate.
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA.
M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain.
The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase to control Viral mRNA splicing.
MA1-082 was used in western blot to determine viral mRNA splicing controlled by the recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase
|Fournier G,Chiang C,Munier S,Tomoiu A,Demeret C,Vidalain PO,Jacob Y,Naffakh N||PLoS pathogens (10:null)||2014|
Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.
MA1-082 was used in western blot to isolate and characterize a human antibody to influenza virus matrix protein 2 (M2e)
|Ozawa T,Jin A,Tajiri K,Takemoto M,Okuda T,Shiraki K,Kishi H,Muraguchi A||Antiviral research (91:283)||2011|
Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes.
MA1-082 was used in western blot to study the role of matrix protein 2 of influenza A virus in blocking autophagosome fusion with lysosomes
|Gannagé M,Dormann D,Albrecht R,Dengjel J,Torossi T,Rämer PC,Lee M,Strowig T,Arrey F,Conenello G,Pypaert M,Andersen J,García-Sastre A,Münz C||Cell host and microbe (6:367)||2009|
Increased immunogenicity and protective efficacy of influenza M2e fused to a tetramerizing protein.
MA1-082 was used in flow cytometry to generate a tetrameric form of the influenza A M2e protein ectodomain with increased immunogenicity and protective efficacy
|Andersson AM,Håkansson KO,Jensen BA,Christensen D,Andersen P,Thomsen AR,Christensen JP||PloS one (7:null)||2012|