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|Tested species reactivity||Virus|
|Published species reactivity||Virus, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||M2 protein from A/WSN/33-infected CV1 cell lysate.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Immunoprecipitation (IP)||10 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-082 detects the N-terminal of the influenza A virus M2 protein.
MA1-082 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~15 kDa protein representing the M2 protein. MA1-082 has demonstrated the ability to immunoprecipitate M2 from [35s] cysteine-labeled WSN-infected cells.
MA1-082 has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain.
The MA1-082 immunogen is M2 protein from A/WSN/33-infected CV1 cell lysate.
Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA.
M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain.
The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased immunogenicity and protective efficacy of influenza M2e fused to a tetramerizing protein.
MA1-082 was used in flow cytometry to generate a tetrameric form of the influenza A M2e protein ectodomain with increased immunogenicity and protective efficacy
|Andersson AM,Håkansson KO,Jensen BA,Christensen D,Andersen P,Thomsen AR,Christensen JP||PloS one (7:null)||2012|
Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.
MA1-082 was used in western blot to isolate and characterize a human antibody to influenza virus matrix protein 2 (M2e)
|Ozawa T,Jin A,Tajiri K,Takemoto M,Okuda T,Shiraki K,Kishi H,Muraguchi A||Antiviral research (91:283)||2011|
Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes.
MA1-082 was used in western blot to study the role of matrix protein 2 of influenza A virus in blocking autophagosome fusion with lysosomes
|Gannagé M,Dormann D,Albrecht R,Dengjel J,Torossi T,Rämer PC,Lee M,Strowig T,Arrey F,Conenello G,Pypaert M,Andersen J,García-Sastre A,Münz C||Cell host & microbe (6:367)||2009|