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Western blot analysis of Influenza A NS2 using 50µg of A) mock DF-1 cell lysates and B) WSN-infected DF-1 cell lysates. Samples were loaded onto a 15% SDS-PAGE gel and probed with an Influenza A NS2 polyclonal antibody (Product # PA5-32234) at a dilution of 1:5000.
|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of influenza A virus NS2 (A/Puerto Rico/8/1934(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-32234 targets Influenza A Virus NS2 in WB applications and shows reactivity with Virus samples.
The PA5-32234 immunogen is recombinant protein fragment of influenza A virus NS2 (A/Puerto Rico/8/1934(H1N1)).
Influenza A viruses are single stranded, negative sense RNA viruses. Two distinct non-structural proteins NS1 and NS2 are translated from one of the eight RNA segments by using different reading frames.The influenza A virus NS2 is located in the nucleus and the cytoplasm. NS2 is essential for nuclear export of influenza virus ribonucleoprotein (RNP) complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Infection of Mouse Macrophages by Seasonal Influenza Viruses Can Be Restricted at the Level of Virus Entry and at a Late Stage in the Virus Life Cycle.
PA5-32234 was used in western blot to study infection of macrophages by influenza A viruses
|Londrigan SL,Short KR,Ma J,Gillespie L,Rockman SP,Brooks AG,Reading PC||Journal of virology (89:12319)||2015|