Immunofluorescent analysis of Influenza A PA in A/WSN/33 infected Vero cells. A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min and stained using an Influenza A PA polyclonal antibody (Product # PA5-32223) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
|Tested species reactivity||Virus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of influenza A virus PA (A/Puerto Rico/8/1934(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32223 targets Influenza A Virus PA in WB and IF applications and shows reactivity with Virus samples.
The PA5-32223 immunogen is recombinant protein fragment of influenza A virus PA (A/Puerto Rico/8/1934(H1N1)).
Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. Recent reports indicate the involvement of PA in 'cap-snatching' mechanism via its endonuclease activity which is essential for cRNA-dependent viral RNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.