Immunofluorescent analysis of Influenza A Virus NS1 in A/WSN/33 infected Vero cells. A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min and stained using an Influenza A Virus NS1 polyclonal antibody (Product # PA5-32243) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C, 30 min.
|Tested species reactivity||Virus|
|Published species reactivity||Virus, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment of Influenza A virus NS1 (A/WSN/1933(H1N1))|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32243 targets Influenza A Virus NS1 in Western blot, immunocytochemistry, and immunofluorescence applications and shows reactivity with Virus samples.
The PA5-32243 immunogen is recombinant protein fragment of Influenza A virus NS1 (A/WSN/1933(H1N1)).
Influenza A viruses are single stranded, negative sense RNA viruses. Two distinct non-structural proteins NS1 and NS2 are translated from one of the eight RNA segments by using different reading frames.The influenza A virus NS2 is located in the nucleus and the cytoplasm. NS2 is essential for nuclear export of influenza virus ribonucleoprotein (RNP) complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro.
PA5-32243 was used in immunocytochemistry to evaluate the differential responses to H3N2 influenza virus infection in vitro in human nasal epithelial cells derived from multiple subjects
|Yan Y,Tan KS,Li C,Tran T,Chao SS,Sugrue RJ,Shi L,Chow VT,Wang DY||The Journal of allergy and clinical immunology (138:276)||2016|
Infection of Mouse Macrophages by Seasonal Influenza Viruses Can Be Restricted at the Level of Virus Entry and at a Late Stage in the Virus Life Cycle.
PA5-32243 was used in western blot to study infection of macrophages by influenza A viruses
|Londrigan SL,Short KR,Ma J,Gillespie L,Rockman SP,Brooks AG,Reading PC||Journal of virology (89:12319)||2015|