|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: P(216) C V L D L K M G T R Q H G D D A S(233).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-906 detects inositol-hexakisphosphate kinase (IP6 kinase) from rat tissues.
PA1-906 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing IP6 kinase from rat brain extract.
PA1-906 immunizing peptide corresponds to amino acid residues 216-233 from human IP6 kinase. PA1-906 immunizing peptide (Cat. # PEP-085) is available for use in neutralization and control experiments.
Inositol 1,4,5-trisphosphate (IP3) is a second messenger for many growth factors, hormones, and neurotransmitters and, upon binding to the IP3 receptor (IP3R), triggers the release of intracellular, luminal calcium to the cytosol. Several additional cellular forms of inositol phosphates have been discovered. These include some which have been recently discovered and characterized to possess up to seven or eight phosphate groups, including pyrophosphates (PP-IPs: pyrophosphate-inositol phosphates). It has been proposed that high-energy pyrophosphates may participate in protein phosphorylation. Inositol-hexakisphosphate (IP6) kinase, or PiUS (Pi Uptake Stimulator) has ATP synthase activity and transfers a phosphate from PP-IP5 to ADP to form ATP thus suggesting an important physiological role for PP-IP5 and IP6 kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification and characterization of a novel inositol hexakisphosphate kinase.
PA1-906 was used in western blot to characterize a new isoform of inositol-hexakisphosphate kinase and investigate its expression and cellular distribution
|Saiardi A,Nagata E,Luo HR,Snowman AM,Snyder SH||The Journal of biological chemistry (276:39179)||2001|