Immunofluorescent analysis of Insulin in human pancreatic tissue using an Insulin recombinant rabbit monoclonal antibody (Product # 701265) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) and nuclei were stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Insulin is a hormone secreted by islet cells of the pancreas. It functions to regulate of blood glucose homeostasis, and modulate lipid metabolism. Insulin is a cleavage product produced from proinsulin produced by beta cells. Proinsulin is cleaved into three peptides. The A and B-chains are covalently linked by disulphide bonds, and make up the insulin molecule.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.