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Immunofluorescent analysis of Insulin in human pancreatic tissue using an Insulin recombinant rabbit monoclonal antibody (Product # 701265) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) and nuclei were stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (IHC)||5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Insulin is a hormone secreted by islet cells of the pancreas. It functions to regulate of blood glucose homeostasis, and modulate lipid metabolism. Insulin is a cleavage product produced from proinsulin produced by beta cells. Proinsulin is cleaved into three peptides. The A and B-chains are covalently linked by disulphide bonds, and make up the insulin molecule.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IRDN, IDDM2, MODY10, ILPR
insulin, preproinsulin, proinsulin, ILPR, IRDN, INS, Proinsulin