|Tested species reactivity||Human, Pig, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||INS04 + INS05|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12042 targets Insulin (INS04 + INS05) in IHC (P) applications and shows reactivity with Human, Porcine, and Rat samples.
The MA5-12042 immunogen is insulin protein.
Insulin is a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell of islets of Langerhans and tumor (insulinoma) marker.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Enteroendocrine cells are a potential source of serum autotaxin in men.
MA5-12042 was used in immunohistochemistry - paraffin section to discover a potential source of serum autotaxin in men by analyzing enteroendocrine cells
|Bolier R,Tolenaars D,Kremer AE,Saris J,Parés A,Verheij J,Bosma PJ,Beuers U,Oude Elferink RP||Biochimica et biophysica acta (1862:696)||2016|
The potential role of combined antioxidant treatment on pancreas of STZ-diabetic mice.
MA5-12042 was used in immunohistochemistry to study mitochondrial and cytosolic oxidative stress in streptozotocin-diabetic mouse pancreas and the effect of combined antioxidant therapy
|Karatug A,Bolkent S||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (65:255)||2013|
Immunohistochemical expression of rabphilin-3A-like (Noc2) in normal and tumor tissues of human endocrine pancreas.
MA5-12042 was used in immunohistochemistry to study Noc2 expression in normal and tumor tissues of the human endocrine pancreas
|Shanmugam C,Katkoori VR,Jhala NC,Grizzle WE,Manne U||Biotechnic and histochemistry : official publication of the Biological Stain Commission (84:39)||2009|
Antidiabetic effects of dipeptidyl peptidase-IV inhibitors and sulfonylureas in streptozotocin-nicotinamide-induced mildly diabetic mice.
MA5-12042 was used in immunohistochemistry to study the antidiabetic effect of DPP-IV inhibitors and sulfoylureas in mildly diabetic mice
|Matsuyama-Yokono A,Tahara A,Nakano R,Someya Y,Shiraki K,Hayakawa M,Shibasaki M||Metabolism: clinical and experimental (58:379)||2009|
Extracellular ATP and zinc are co-secreted with insulin and activate multiple P2X purinergic receptor channels expressed by islet beta-cells to potentiate insulin secretion.
MA5-12042 was used in immunohistochemistry to examine the role of extracellular ATP and zinc in insulin secretion
|Richards-Williams C,Contreras JL,Berecek KH,Schwiebert EM||Purinergic signalling (4:393)||2008|
Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes.
MA5-12042 was used in immunohistochemistry to study the antioxidant and pancreas-protective effect of aucubin in streptozotocin-induced diabetes
|Jin L,Xue HY,Jin LJ,Li SY,Xu YP||European journal of pharmacology (582:162)||2008|
Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells.
MA5-12042 was used in immunocytochemistry to study the transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells
|Zhu XQ,Pan XH,Wang W,Chen Q,Pang RQ,Cai XM,Hoffman AR,Hu JF||Biomaterials (31:2779)||2010|