Immunohistochemistry analysis of CD41 was performed on equine endometrial tissue sections. 5-µm-thick sections were prepared from formalin-fixed paraffin-embedded tissue blocks and were dried on slide plate at 37°C overnight. The sections were placed in a Bond Max Automated Immunohistochemistry Vision Biosystem (Leica Microsystems GmbH, Wetzlar, Germany) according to the following protocol. First, tissues were deparaffinized and pre-treated with Epitope Retrieval Solution 1 (Citrate based pH6) at 98°C for 10 minutes. After washing steps, peroxidase blocking was carried out for 10 min using the Bond Polymer Refine Detection Kit DC9800 (Leica Microsystems GmbH). Tissues were again washed and then incubated with the primary antibody (Product # PA5-22307, 1:200) for 30 min then the secondary antibody diluted 1:1000. Subsequently, tissues were incubated with polymer for 10 min and developed with DAB-Chromogen for 10 min. Data courtesy of Antibody Data Exchange Program.
|Tested species reactivity||Horse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 210 and 532 of Integrin alpha 2b (Uniprot ID#P08514)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22307 targets CD41/Integrin alpha 2b in Western blot and immunohistochemistry (paraffin) applications and shows reactivity with Human samples.
PA5-22307 detects ITGA2b (CD41) protein in formalin-fixed paraffin-embedded equine endometrial tissue.
The PA5-22307 immunogen is a recombinant fragment corresponding to a region within amino acids 210 and 532 of Integrin alpha 2b (Uniprot ID#P08514).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.