|Tested species reactivity||Many|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||JM leukemia line.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1µg/10^5 cells|
|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
Clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. This antibody can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1.
This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays.
CD49d, also known as integrin alpha-4 or VLA-4 subunit alpha, is a ~150kDa single pass type 1 transmembrane glycoprotein that contains seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue through its interaction with MadCam-1, preferentially expressed on Peyerâ€™s patch high endothelial venules and postcapillary venules in lamina propria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
¿-Integrin expression and function modulates presentation of cell surface calreticulin.
MA1-80529 was used in immunoprecipitation to determine modulation of presentation of cell surfaced calreticulin by alpha-integrin function and expression
|Liu CC,Leclair P,Monajemi M,Sly LM,Reid GS,Lim CJ||Cell death and disease (7:null)||2016|