Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of HeLa cells stained with an ITGA6 polyclonal antibody (Product # PA5-12334). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with an ITGA6 polyclonal antibody (Product # PA5-12334) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 1043-1072 amino acids from human ITGA6|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken based on sequence homology.
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CD49 antigen-like family member F; CD49f; integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B
CD49f; ITGA6; ITGA6B; VLA-6