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MUbio
Integrin alpha 6A/CD49f Monoclonal Antibody (NKI-GoH3)
Product Details
MUB0908P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
NKI-GoH3
Immunogen
Integrin alpha 6A / CD49f
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The epitope recognized by GoH3 is located on glycoprotein Ic or the a6 subunit of integrin. This alpha-6 (glycoprotein Ic) is expressed as a physical complex with glycoprotein IIa within the plasma membrane protein on platelets and with the b4 subunit of integrin on normal epithelial cells and (colon) carcinoma cells, peripheral nerves, and endohelia. The epitope of the monoclonal antibody is still available when the Ic glycoprotein is within the complex.
GoH3 stains the basement membrane zone in skin sections, the myoepithelial cells in the mammary gland, tubules in kidney sections, acini and ducts in salivary glands, the epithelial layer in colonic crypts, the perineurium and the Schwann cells in sections of Human peripheral nerves. Smooth muscle cells, endothelial cells and striated muscle and the muscularis of the large intestine are not stained.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6
Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6
UniProt ID: (Human) P23229, (Mouse) Q61739
Entrez Gene ID: (Human) 3655, (Mouse) 16403
cell-substrate junction assembly
cell-matrix adhesion
integrin-mediated signaling pathway
ectodermal cell differentiation
positive regulation of cell-cell adhesion
extracellular matrix organization
hemidesmosome assembly
cell-substrate adhesion
cellular response to extracellular stimulus
cell adhesion mediated by integrin
nail development
positive regulation of phosphorylation
positive regulation of apoptotic process
positive regulation of GTPase activity
skin development
positive regulation of transcription from RNA polymerase II promoter
filopodium assembly
digestive tract development
brown fat cell differentiation
leukocyte migration
renal system development
amelogenesis
cell-cell adhesion
negative regulation of extrinsic apoptotic signaling pathway
cell adhesion
positive regulation of cell-substrate adhesion
single organismal cell-cell adhesion
odontogenesis of dentin-containing tooth
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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