The epitope recognized by GoH3 is located on glycoprotein Ic or the a6 subunit of integrin. This alpha-6 (glycoprotein Ic) is expressed as a physical complex with glycoprotein IIa within the plasma membrane protein on platelets and with the b4 subunit of integrin on normal epithelial cells and (colon) carcinoma cells, peripheral nerves, and endohelia. The epitope of the monoclonal antibody is still available when the Ic glycoprotein is within the complex.
GoH3 stains the basement membrane zone in skin sections, the myoepithelial cells in the mammary gland, tubules in kidney sections, acini and ducts in salivary glands, the epithelial layer in colonic crypts, the perineurium and the Schwann cells in sections of Human peripheral nerves. Smooth muscle cells, endothelial cells and striated muscle and the muscularis of the large intestine are not stained.
The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha6p; CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; integrin alpha6B; integrin, alpha 6; Processed integrin alpha-6; VLA-6
Gene Aliases: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6
Molecular Function: integrin