Western blot analysis of Integrin alpha-7 using Integrin alpha-7 polyclonal antibody (Product # PA5-37435) at a dilution of 1:500. Lane 1: Hela cell lysate treated with LPS, Lane 2: Raw264.7 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV, Lane 4: Hela cell lysate, Lane 5: Mouse heart tissue lysate, Lane 6: PC12 cell lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 926-976 of human Integrin alpha7|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 25 and 129 kDa.
Purity is >95% by SDS-PAGE.
Integrins are heterodimers composed of noncovalently associated transmembrane alpha and beta subunits. The 16 alpha and 8 beta subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrinmediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.