Immunofluorescent analysis of CD11c (green) showing staining in the cytoplasm and membrane of THP-1 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD11c monoclonal antibody (Product # MA11C5) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||Mouse p150,95 (CD11c)|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA11C5 targets CD11c in FACS, IHC, ICC/IF and IP applications and shows reactivity with mouse samples. This antibody does not react with human HL-60 cells in FACS applications.
The MA11C5 immunogen is mouse p150,95 (CD11c).
MA11C5 detects CD11c which has a predicted molecular weight of approximately 126 kDa.
This product is a Low Endotoxin formulation.
This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of Signal Transducer and Activator of Transcription 3 (STAT3) reduces neonatal hypoxic-ischaemic brain damage.
MA11C5 was used in immunohistochemistry - frozen section to study the role of STAT3 in hypoxia ischemia-induced brain damage
|Hristova M,Rocha-Ferreira E,Fontana X,Thei L,Buckle R,Christou M,Hompoonsup S,Gostelow N,Raivich G,Peebles D||Journal of neurochemistry (136:981)||2016|
Maternal immune stimulation during pregnancy shapes the immunological phenotype of offspring.
MA11C5 was used in flow cytometry to use poly(I:C)-injected dams to study the effects of inflammation on fetal development.
|Mandal M,Donnelly R,Elkabes S,Zhang P,Davini D,David BT,Ponzio NM||Brain, behavior, and immunity (33:33)||2013|
TIM-3 regulates innate immune cells to induce fetomaternal tolerance.
MA11C5 was used in flow cytometry to determine the role of TIM-3-expressing innate immune cells in the regulation of tolerance at the fetomaternal interface.
|Chabtini L,Mfarrej B,Mounayar M,Zhu B,Batal I,Dakle PJ,Smith BD,Boenisch O,Najafian N,Akiba H,Yagita H,Guleria I||Journal of immunology (Baltimore, Md. : 1950) (190:88)||2013|
Cigarette smoke exposure impairs dendritic cell maturation and T cell proliferation in thoracic lymph nodes of mice.
MA11C5 was used in flow cytometry to examine the consequences of cigarette smoke exposure on DC function in mice.
|Robbins CS,Franco F,Mouded M,Cernadas M,Shapiro SD||Journal of immunology (Baltimore, Md. : 1950) (180:6623)||2008|
Loss of Gadkin Affects Dendritic Cell Migration In Vitro.
MA11C5 was used in flow cytometry to determine the affects of dendritic cell migration in vitro by loss of gadkin
|Schachtner H,Weimershaus M,Stache V,Plewa N,Legler DF,Höpken UE,Maritzen T||PloS one (10:null)||2015|
In vivo transcriptional profiling of Yersinia pestis reveals a novel bacterial mediator of pulmonary inflammation.
MA11C5 was used in flow cytometry to characterize Yersinia pestis as a novel bacterial mediator of pulmonary inflammation due to in vivo transcriptional profiling
|Pechous RD,Broberg CA,Stasulli NM,Miller VL,Goldman WE||mBio (6:e02302)||2015|
Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.
MA11C5 was used in flow cytometry to assess the contribution of Tbx1 to thymus and parathyroid development
|Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER||Development (Cambridge, England) (141:2950)||2014|
|Not Applicable||Not Cited||
Early host cell targets of Yersinia pestis during primary pneumonic plague.
MA11C5 was used in flow cytometry to determine when in infection Yersinia pestis infects macrophages and neutrophils
|Pechous RD,Sivaraman V,Price PA,Stasulli NM,Goldman WE||PLoS pathogens (9:null)||2013|
|Not Applicable||Not Cited||
Diesel exhaust particles stimulate adaptive immunity by acting on pulmonary dendritic cells.
MA11C5 was used in flow cytometry to determine if diesel exhaust particles affect dendritic cell function
|Provoost S,Maes T,Willart MA,Joos GF,Lambrecht BN,Tournoy KG||Journal of immunology (Baltimore, Md. : 1950) (184:426)||2010|
|Not Applicable||Not Cited||
Respiratory Francisella tularensis live vaccine strain infection induces Th17 cells and prostaglandin E2, which inhibits generation of gamma interferon-positive T cells.
MA11C5 was used in flow cytometry to investigate how the route of inoculation with Francisella tularensis influences the primary acute adaptive immune response
|Woolard MD,Hensley LL,Kawula TH,Frelinger JA||Infection and immunity (76:2651)||2008|
Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.
MA11C5 was used in flow cytometry to determine the expression of CD39 in mouse and human T cells
|Borsellino G,Kleinewietfeld M,Di Mitri D,Sternjak A,Diamantini A,Giometto R,Höpner S,Centonze D,Bernardi G,Dell'Acqua ML,Rossini PM,Battistini L,Rötzschke O,Falk K||Blood (110:1225)||2007|
|Not Applicable||Not Cited||
Time-course expression of Toll-like receptors 2 and 4 in inflammatory bowel disease and homeostatic effect of VIP.
MA11C5 was used in flow cytometry to study TLR2 and 4 expression in a trinitrobenzene sulfonic acid-induced colitis model of Crohn's disease
|Gomariz RP,Arranz A,Abad C,Torroba M,Martinez C,Rosignoli F,Garcia-Gómez M,Leceta J,Juarranz Y||Journal of leukocyte biology (78:491)||2005|
Immunization with the adjuvant MF59 induces macrophage trafficking and apoptosis.
MA11C5 was used in flow cytometry to investigate the effect of MF59 for the migration and survival of macrophages
|Dupuis M,Denis-Mize K,LaBarbara A,Peters W,Charo IF,McDonald DM,Ott G||European journal of immunology (31:2910)||2001|
Antigen presentation function of brain-derived dendriform cells depends on astrocyte help.
MA11C5 was used in flow cytometry to investigate the roles of GM-CSF and M-CSF in antigen presentation function in brains
|Fischer HG,Bielinsky AK||International immunology (11:1265)||1999|
Generation of tumor immunity by bone marrow-derived dendritic cells correlates with dendritic cell maturation stage.
MA11C5 was used in flow cytometry to investigate the induction of tumor immunity by dendritic cells at different stages
|Labeur MS,Roters B,Pers B,Mehling A,Luger TA,Schwarz T,Grabbe S||Journal of immunology (Baltimore, Md. : 1950) (162:168)||1999|
Ultraviolet light-induced immune tolerance is mediated via the Fas/Fas-ligand system.
MA11C5 was used in flow cytometry to investigate the mechanism for the immune tolerance induced by UV stimulation
|Schwarz A,Grabbe S,Grosse-Heitmeyer K,Roters B,Riemann H,Luger TA,Trinchieri G,Schwarz T||Journal of immunology (Baltimore, Md. : 1950) (160:4262)||1998|
CD14 is expressed by subsets of murine dendritic cells and upregulated by lipopolysaccharide.
MA11C5 was used in flow cytometry to investigate the expression of CD14 in murine dendritic cells and its regulation by lipopolysaccharide
|Mahnke K,Becher E,Ricciardi-Castagnoli P,Luger TA,Schwarz T,Grabbe S||Advances in experimental medicine and biology (417:145)||1997|
The pathogenesis of severe fever with thrombocytopenia syndrome virus infection in alpha/beta interferon knockout mice: insights into the pathologic mechanisms of a new viral hemorrhagic fever.
MA11C5 was used in immunohistochemistry to study severe fever with thrombocytopenia syndrome virus infection pathogenesis using a murine model
|Liu Y,Wu B,Paessler S,Walker DH,Tesh RB,Yu XJ||Journal of virology (88:1781)||2014|
|Not Applicable||Not Cited||
The location of splenic NKT cells favours their rapid activation by blood-borne antigen.
MA11C5 was used in immunohistochemistry to study splenic natural killer T cells
|Barral P,Sánchez-Niño MD,van Rooijen N,Cerundolo V,Batista FD||The EMBO journal (31:2378)||2012|
The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain.
MA11C5 was used in immunohistochemistry to investigate the role of the ATM cofactor ATMIN in the aging brain
|Kanu N,Penicud K,Hristova M,Wong B,Irvine E,Plattner F,Raivich G,Behrens A||The Journal of biological chemistry (285:38534)||2010|
CD11c/EYFP transgene illuminates a discrete network of dendritic cells within the embryonic, neonatal, adult, and injured mouse brain.
MA11C5 was used in immunohistochemistry to investigate dendritic cells within the central nervous system of CD11c/EYFP transgenic mice
|Bulloch K,Miller MM,Gal-Toth J,Milner TA,Gottfried-Blackmore A,Waters EM,Kaunzner UW,Liu K,Lindquist R,Nussenzweig MC,Steinman RM,McEwen BS||The Journal of comparative neurology (508:687)||2008|
Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.
MA11C5 was used in immunohistochemistry to investigate the diversity of macrophages in mouse spleen and liver
|Lloyd CM,Phillips AR,Cooper GJ,Dunbar PR||Journal of immunological methods (334:70)||2008|
Type I interferon dependence of plasmacytoid dendritic cell activation and migration.
MA11C5 was used in immunohistochemistry to investigate the role of inteferons during the stimulation of dendritic cells
|Asselin-Paturel C,Brizard G,Chemin K,Boonstra A,O'Garra A,Vicari A,Trinchieri G||The Journal of experimental medicine (201:1157)||2005|
Study on pancreatic lymphatics in nonobese diabetic mouse with prevention of insulitis and diabetes by adjuvant immunotherapy.
MA11C5 was used in immunohistochemistry to investigate the effect of CFA for the regulation of pancreatic lymphatics in NOD mice
|Qu P,Ji RC,Shimoda H,Miura M,Kato S||The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology (281:1326)||2004|
Identification of a novel macrophage population in the normal mouse corneal stroma.
MA11C5 was used in immunohistochemistry to investigate the types of cells in the normal corneal stroma
|Brissette-Storkus CS,Reynolds SM,Lepisto AJ,Hendricks RL||Investigative ophthalmology and visual science (43:2264)||2002|
Tissue distribution of products of the mouse decay-accelerating factor (DAF) genes. Exploitation of a Daf1 knock-out mouse and site-specific monoclonal antibodies.
MA11C5 was used in immunohistochemistry to investigate the localization of DAF protein in vivo
|Lin F,Fukuoka Y,Spicer A,Ohta R,Okada N,Harris CL,Emancipator SN,Medof ME||Immunology (104:215)||2001|
The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
MA11C5 was used in immunocytochemistry to investigate the localization of B7-2 in mice
|Inaba K,Witmer-Pack M,Inaba M,Hathcock KS,Sakuta H,Azuma M,Yagita H,Okumura K,Linsley PS,Ikehara S,Muramatsu S,Hodes RJ,Steinman RM||The Journal of experimental medicine (180:1849)||1994|