Flow cytometry analysis of Integrin beta-1 / CD29 was done on U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Integrin beta-1 / CD29 Mouse Monoclonal Antibody (MA513658, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
|Tested species reactivity||Human, Pig|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human melanoma V+B2 cell line|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13658 targets CD29 in FACS, IF, and IHC (F) applications and shows reactivity with Human and Porcine samples.
The MA5-13658 immunogen is human melanoma V+B2 cell line.
CD29 (integrin Beta-1 / gpIIa) is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. The CD29 is expressed at the cell surface exclusively as part of a heterodimer, in association with one of at least nine different integrin alpha subunits (alpha, alpha, alpha, alpha, alpha, alpha, alpha, alpha, and alphav). With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed on nearly all cell and tissue types.
IP-MS enrichment of ITGB1 (LFQ intensity): ITGB1 was enriched 53-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and ITGB1 antibody (Part No. MA5-13658). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
MA5-13658 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium
|Qi H,Zheng X,Yuan X,Pflugfelder SC,Li DQ||Journal of cellular physiology (225:180)||2010|
Characterization of putative stem cell phenotype in human limbal epithelia.
MA5-13658 was used in immunohistochemistry to evaluate the stem cell molecular markers in human limbal epithelia
|Chen Z,de Paiva CS,Luo L,Kretzer FL,Pflugfelder SC,Li DQ||Stem cells (Dayton, Ohio) (22:355)||2004|
Basal cells of second trimester fetal breasts: immunohistochemical study of myoepithelial precursors.
MA5-13658 was used in immunohistochemistry to study myoepithelial precursors in second trimester fetal breasts
|Jolicoeur F,Gaboury LA,Oligny LL||Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society (6:398)||2004|
Telomerase-immortalized non-malignant human prostate epithelial cells retain the properties of multipotent stem cells.
MA5-13658 was used in flow cytometry to examine the totipotency of immortalized non-malignant human prostate epithelial cells
|Li H,Zhou J,Miki J,Furusato B,Gu Y,Srivastava S,McLeod DG,Vogel JC,Rhim JS||Experimental cell research (314:92)||2008|
Identification of putative stem cell markers, CD133 and CXCR4, in hTERT-immortalized primary nonmalignant and malignant tumor-derived human prostate epithelial cell lines and in prostate cancer specimens.
MA5-13658 was used in flow cytometry to examine the stem cell biomarkers of immortalized or cancerous cells
|Miki J,Furusato B,Li H,Gu Y,Takahashi H,Egawa S,Sesterhenn IA,McLeod DG,Srivastava S,Rhim JS||Cancer research (67:3153)||2007|
Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells.
MA5-13658 was used in immunocytochemistry to evaluate gap junction protein connexin 43 as a negative marker of the pluripotency of human limbal epithelial cells
|Chen Z,Evans WH,Pflugfelder SC,Li DQ||Stem cells (Dayton, Ohio) (24:1265)||2006|
Re-arrangements of podosome structures are observed when Hck is activated in myeloid cells.
MA5-13658 was used in immunocytochemistry to study the role of Hck in podosome structures
|Poincloux R,Vincent C,Labrousse A,Castandet J,Rigo M,Cougoule C,Bordier C,Le Cabec V,Maridonneau-Parini I||European journal of cell biology (85:327)||2006|