Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA1805) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 4 publications below|
MA1805 targets CD18 in FACS, IA, and IP applications and shows reactivity with mouse samples.
The MA1805 immunogen is cD18.
MA1805 detects CD18 which has a predicted molecular weight of approximately 83 kDa.
This product is a Low Endotoxin formulation.
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice.
MA1805 was used in blocking/activating experiment to study the role of VLA-4 in CD18-independent neutrophil emigration induced by bacteria
|Tasaka S,Richer SE,Mizgerd JP,Doerschuk CM||American journal of respiratory and critical care medicine (166:53)||2002|
alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes.
MA1805 was used in blocking/activating experiment to investigate the mechanism of heart injury mediated by emigrated PMNs
|Poon BY,Ward CA,Cooper CB,Giles WR,Burns AR,Kubes P||The Journal of cell biology (152:857)||2001|
The hepatic inflammatory response after acetaminophen overdose: role of neutrophils.
MA1805 was used in blocking/activating experiment to test the role of accumulated neutrophils in liver damage
|Lawson JA,Farhood A,Hopper RD,Bajt ML,Jaeschke H||Toxicological sciences : an official journal of the Society of Toxicology (54:509)||2000|
Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr.
MA1805 was used in blocking/activating experiment to investigate the role of Fgr for the modulation of macrophages phagocytosis
|Gresham HD,Dale BM,Potter JW,Chang PW,Vines CM,Lowell CA,Lagenaur CF,Willman CL||The Journal of experimental medicine (191:515)||2000|