Immunofluorescence analysis of CD18 Monoclonal Antibody (TA-4) was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD18 (TA-4) Mouse Monoclonal Antibody (MA1807) at 2ug/ml in 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Rat beta2 Integrin|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1807 targets CD18 in FACS applications and shows reactivity with Rat samples.
The MA1807 immunogen is rat beta2 Integrin.
MA1807 detects CD18 which has a predicted molecular weight of approximately 83 kDa.
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.
MA1807 was used in ELISA to investigate the role of endothelial-cell VCAM-1 in eosinophil accumulation
|Larbi KY,Allen AR,Tam FW,Haskard DO,Lobb RR,Silva PM,Nourshargh S||Blood (96:3601)||2000|
Intratracheal injection of endotoxin and cytokines. IX. Contribution of CD11a/ICAM-1 to neutrophil emigration.
MA1807 was used in blocking/activating experiment to investigate the mechanism of neutrophil emigration induced by lipopolysaccharide and tumor necrosis factor
|Tang WW,Yi ES,Remick DG,Wittwer A,Yin S,Qi M,Ulich TR||The American journal of physiology (269:L653)||1995|