Flow cytometry analysis of Integrin beta-2/CD18 in rat spleen cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with an Integrin beta-2/CD18 monoclonal antibody (Product # MA1-817) at a dilution of 1ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
|Tested species reactivity||Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat beta2 Integrin|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1817 targets CD18 in FACS, IP, and WB applications and shows reactivity with Rat samples.
The MA1817 immunogen is rat beta2 Integrin.
MA1817 detects CD18 which has a predicted molecular weight of approximately 83 kDa.
This product is a Low Endotoxin formulation.
The product of this gene belongs to the integrin beta chain family of proteins. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. This gene encodes the integrin beta chain beta 2. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PR-39, a proline/arginine-rich antimicrobial peptide, exerts cardioprotective effects in myocardial ischemia-reperfusion.
MA1817 was used in flow cytometry to study the effect of a proline/arginine-rich antimicrobial peptide PR-39 on cardic protection
|Ikeda Y,Young LH,Scalia R,Ross CR,Lefer AM||Cardiovascular research (49:69)||2001|
Simvastatin preserves the ischemic-reperfused myocardium in normocholesterolemic rat hearts.
MA1817 was used in flow cytometry to investigate the protective effect of simvastatin on ischemic myocardium
|Lefer AM,Campbell B,Shin YK,Scalia R,Hayward R,Lefer DJ||Circulation (100:178)||1999|
Soluble ICAM-1 activates lung macrophages and enhances lung injury.
MA1817 was used in ELISA to investigate the role of ICAM-1 in lung macrophage activity and lung injury
|Schmal H,Czermak BJ,Lentsch AB,Bless NM,Beck-Schimmer B,Friedl HP,Ward PA||Journal of immunology (Baltimore, Md. : 1950) (161:3685)||1998|