|Tested species reactivity||Human|
|Published species reactivity||Tag, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant human recombinant IFN-gamma produced in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mycophenolic acid differentially impacts B cell function depending on the stage of differentiation.
AHC4032 was used in flow cytometry to elucidate the effect of mycophenolic acid on B cells.
|Karnell JL,Karnell FG,Stephens GL,Rajan B,Morehouse C,Li Y,Swerdlow B,Wilson M,Goldbach-Mansky R,Groves C,Coyle AJ,Herbst R,Ettinger R||Journal of immunology (Baltimore, Md. : 1950) (187:3603)||2011|
Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines.
AHC4032 was used in flow cytometry to report an in vitro differentiation assay in which human naive CD4(+) cells are driven toward either the Th1 or Th2 phenotype and study them.
|Cousins DJ,Lee TH,Staynov DZ||Journal of immunology (Baltimore, Md. : 1950) (169:2498)||2002|
|Vaccine-induced CD8+ T-cell responses to MAGE-3 correlate with clinical outcome in patients with melanoma.||Reynolds SR,Zeleniuch-Jacquotte A,Shapiro RL,Roses DF,Harris MN,Johnston D,Bystryn JC||Clinical cancer research : an official journal of the American Association for Cancer Research (9:657)||2003|
|Human||Not Cited||Human nasopharyngeal-associated lymphoreticular tissues. Functional analysis of subepithelial and intraepithelial B and T cells from adenoids and tonsils.||Boyaka PN,Wright PF,Marinaro M,Kiyono H,Johnson JE,Gonzales RA,Ikizler MR,Werkhaven JA,Jackson RJ,Fujihashi K,Di Fabio S,Staats HF,McGhee JR||The American journal of pathology (157:2023)||2000|