|Tested species reactivity||Pig|
|Published species reactivity||Pig, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant swine interferon-gamma|
|Storage buffer||PBS, pH 7.2|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Heterologous challenge in the presence of maternally-derived antibodies results in vaccine-associated enhanced respiratory disease in weaned piglets.
ASC4032 was used in flow cytometry to study the result of vaccine-associated enhanced respiratory disease in weaned piglets due to heterologous challenge in the presence of maternally-derived antibodies
|Rajao DS,Sandbulte MR,Gauger PC,Kitikoon P,Platt R,Roth JA,Perez DR,Loving CL,Vincent AL||Virology (491:79)||2016|
Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs.
ASC4032 was used in flow cytometry to compare humoral and cellular immune responses to inactivated swine influenza virus vaccine using pigs.
|Platt R,Vincent AL,Gauger PC,Loving CL,Zanella EL,Lager KM,Kehrli ME,Kimura K,Roth JA||Veterinary immunology and immunopathology (142:252)||2011|