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|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant human IFN-gamma, expressed in E. coli.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24779 detects Interferon gamma from human samples.
PA1-24779 has been successfully used in inhibition assay, and Western blot procedures.
The PA1-24779 immunogen is recombinant human IFN-gamma, expressed in E. coli.
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
IFN-gamma; immune interferon; interferon, gamma
IFG; IFI; IFNG