|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified, recombinant rat IFNG, expressed in E. coli.|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-1 µg/ml|
|Inhibition Assays (IA)||0.08-0.20 ug/ml|
|Immunohistochemistry (IHC)||0.5-5 µg/ml|
|Neutralization (Neu)||0.08-0.20 ug/ml|
|Western Blot (WB)||0.1-0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-24782 detects Interferon gamma from mouse and rat samples. PA1-24782 will not react with human.
PA1-24782 has been successfully used in Western blot, ELISA, neutralization and immunohistochemistry procedures. The Neutralization Dose 50 (ND50) for anti-rat IFNG is approximately 0.08 to 0.2 ug/ml in the presence of 2.5 ng/ml of recombinant rat IFNG, measuring the protection of mouse L-929 cells from the lytic effect of the virus.
The PA1-24782 immunogen is purified, recombinant rat IFNG, expressed in E. coli.
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased CCN2, substance P and tissue fibrosis are associated with sensorimotor declines in a rat model of repetitive overuse injury.
PA1-24782 was used in immunohistochemistry and western blot to examine the mechanism of sensorimotor declines in a rat model of repetitive overuse injury
|Fisher PW,Zhao Y,Rico MC,Massicotte VS,Wade CK,Litvin J,Bove GM,Popoff SN,Barbe MF||Journal of cell communication and signaling (9:37)||2015|