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|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Eubacteria, Mouse|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Recombinant mouse IFN-gamma|
|Storage buffer||PBS with 0.1% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of a Novel Human Leukocyte Antigen-DQA1*01:02;DRB1*15:01 Mixed Isotype Heterodimer in the Pathogenesis of "Humanized" Multiple Sclerosis-like Disease.
AMC4834 was used in ELISA to assess the impact of the different HLA-DR15 haplotype alleles on disease susceptibility in a new humanized multiple sclerosis model
|Kaushansky N,Eisenstein M,Boura-Halfon S,Hansen BE,Nielsen CH,Milo R,Zeilig G,Lassmann H,Altmann DM,Ben-Nun A||The Journal of biological chemistry (290:15260)||2015|
DQB1*0602 rather than DRB1*1501 confers susceptibility to multiple sclerosis-like disease induced by proteolipid protein (PLP).
AMC4834 was used in ELISA to use 'HLA-humanized' mice to determine the contribution of DRB*50 and DQB*0602 alleles to susceptibility to autoimmunity.
|Kaushansky N,Altmann DM,David CS,Lassmann H,Ben-Nun A||Journal of neuroinflammation (9:null)||2012|
|A multiepitope subunit vaccine conveys protection against extraintestinal pathogenic Escherichia coli in mice.||Wieser A,Romann E,Magistro G,Hoffmann C,Nörenberg D,Weinert K,Schubert S||Infection and immunity (78:3432)||2010|
|Mouse||Not Cited||HLA-DQB1*0602 determines disease susceptibility in a new "humanized" multiple sclerosis model in HLA-DR15 (DRB1*1501;DQB1*0602) transgenic mice.||Kaushansky N,Altmann DM,Ascough S,David CS,Lassmann H,Ben-Nun A||Journal of immunology (Baltimore, Md. : 1950) (183:3531)||2009|
|Mouse||Not Cited||Yersinia outer protein P inhibits CD8 T cell priming in the mouse infection model.||Trülzsch K,Geginat G,Sporleder T,Ruckdeschel K,Hoffmann R,Heesemann J,Rüssmann H||Journal of immunology (Baltimore, Md. : 1950) (174:4244)||2005|
Multiantigen/multiepitope-directed immune-specific suppression of "complex autoimmune encephalomyelitis" by a novel protein product of a synthetic gene.
AMC4834 was used in ELISA to perform a pilot study testing if concomitantly inhibiting pathogenic anti-myelin T cell reactivities by tolerogenic administration of an artificial multiantigen/multiepitope protein is beneficial.
|Zhong MC,Kerlero de Rosbo N,Ben-Nun A||The Journal of clinical investigation (110:81)||2002|
The putative natural killer decoy early gene m04 (gp34) of murine cytomegalovirus encodes an antigenic peptide recognized by protective antiviral CD8 T cells.
AMC4834 was used in ELISA to study how MCMV escapes detection by the host immune system.
|Holtappels R,Thomas D,Podlech J,Geginat G,Steffens HP,Reddehase MJ||Journal of virology (74:1871)||2000|
Prevention of neonatal tolerance by a plasmid encoding granulocyte-macrophage colony stimulating factor.
AMC4834 was used in ELISA to optimize neonate vaccination using DNA encoding the circumsporozoite protein of malaria.
|Ishii KJ,Weiss WR,Klinman DM||Vaccine (18:703)||1999|
Active recruitment of DNA methyltransferases regulates interleukin 4 in thymocytes and T cells.
AMC4834 was used in blocking or activating experiment to investigate how T cells regulate interleukin 4 expression.
|Makar KW,Pérez-Melgosa M,Shnyreva M,Weaver WM,Fitzpatrick DR,Wilson CB||Nature immunology (4:1183)||2003|
gamma interferon; IFN-gamma; IFN-y; IFNG; interferon gamma; Interferon y
Ifg; IFN-g; Ifng