|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||E.coli-expressed recombinant mouse IFN gamma|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.1-0.25 µg per 1x10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
RORgamma-expressing Th17 cells induce murine chronic intestinal inflammation via redundant effects of IL-17A and IL-17F.
RM9004 was used in flow cytometry to ascertain the roles of Th17 cells and retinoic acid receptor-related organ receptor gamma in chronic intestinal inflammation
|Leppkes M,Becker C,Ivanov II,Hirth S,Wirtz S,Neufert C,Pouly S,Murphy AJ,Valenzuela DM,Yancopoulos GD,Becher B,Littman DR,Neurath MF||Gastroenterology (136:257)||2009|
Prevention of graft-versus-host disease by a novel immunosuppressant, PG490-88, through inhibition of alloreactive T cell expansion.
RM9004 was used in flow cytometry to discuss PG490-88.
|Chen BJ,Liu C,Cui X,Fidler JM,Chao NJ||Transplantation (70:1442)||2000|