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|Tested species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified recombinant human interferon-gamma (IFN-gamma).|
|Storage buffer||PBS, pH 7.5|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product is recommended as a capture antibody in Sandwich ELISA applications.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel ELISPOT assay to enhance detection of antigen-specific T cells employing antigen-presenting cells expressing vector-driven human B7-1.
||Arlen PM,Gulley JL,Palena C,Marshall J,Schlom J,Tsang KY||Journal of immunological methods (279:183)||2003|
Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.
||Mascart F,Verscheure V,Malfroot A,Hainaut M,Piérard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C||Journal of immunology (Baltimore, Md. : 1950) (170:1504)||2003|
Biological characterization of lipopolysaccharide from Treponema pectinovorum.
||Kesavalu L,Falk CW,Davis KJ,Steffen MJ,Xu X,Holt SC,Ebersole JL||Infection and immunity (70:211)||2002|
Cytokine responses to treponema pectinovorum and treponema denticola in human gingival fibroblasts.
||Nixon CS,Steffen MJ,Ebersole JL||Infection and immunity (68:5284)||2000|
Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor beta1 and beta2 chain expression.
AHC4432 was used in ELISA to show that cholera toxin inhibits the production of interleukin-2.
|Braun MC,He J,Wu CY,Kelsall BL||The Journal of experimental medicine (189:541)||1999|
Flavopiridol-related proinflammatory syndrome is associated with induction of interleukin-6.
||Messmann RA,Ullmann CD,Lahusen T,Kalehua A,Wasfy J,Melillo G,Ding I,Headlee D,Figg WD,Sausville EA,Senderowicz AM||Clinical cancer research : an official journal of the American Association for Cancer Research (9:562)||2003|
IFN-gamma, immune interferon, interferon gamma, IFN-y, Interferon y, IFNG