|Tested species reactivity||Avian, Dog, Human, Non-human primate, Pig, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native human protein.|
|Storage buffer||PBS, pH 7.4, with 40% glycerol|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg|
|Western Blot (WB)||1 - 2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25753 detects Involucrin from human, rat, canine, gorilla, monkey, owl, and porcine samples.
MA1-25753 has been successfully used in ELISA, IF/ICC, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures.
The MA1-25753 immunogen is purified, full length, native human protein. The epitope has been mapped between residues 421-568 of human involucrin.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.