|Tested species reactivity||Dog, Human, Non-human primate, Pig, Rat|
|Published species reactivity||Rat, Sheep, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human keratinocytes' involucrin|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-200|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11800 targets Involucrin in ELISA, IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, Non-human primate, Porcine, and Rat samples.
The MA5-11800 immunogen is human keratinocytes' involucrin.
Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Insulin-transferrin-selenium as an alternative to foetal serum for epidermal equivalents.
MA5-11800 was used in immunohistochemistry to study the use of a defined medium containing insulin, transferrin and selenium as an alternative to fetal calf serum for the culture of epidermal equivalents
|Mainzer C,Barrichello C,Debret R,Remoué N,Sigaudo-Roussel D,Sommer P||International journal of cosmetic science (36:427)||2014|
Optimizing in vitro culture conditions leads to a significantly shorter production time of human dermo-epidermal skin substitutes.
MA5-11800 was used in immunohistochemistry to develop a culture system for the rapid generation of high quality human autologous dermo-epidermal skin substitutes
|Pontiggia L,Klar A,Böttcher-Haberzeth S,Biedermann T,Meuli M,Reichmann E||Pediatric surgery international (29:249)||2013|
Saurian papulosis: a new clinicopathological entity.
MA5-11800 was used in immunohistochemistry to characterize two clinical cases of saurian papulosis
|Molina-Ruiz AM,del Carmen Fariña M,Carrasco L,Santonja C,Rodríguez-Peralto JL,Torrelo A,Kutzner H,Requena L||Journal of the American Academy of Dermatology (68:e17)||2013|
Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium.
MA5-11800 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth
|Mitchell D,Israr M,Alam S,Kishel J,Dinello D,Meyers C||HIV medicine (13:276)||2012|
CD44 regulates tight-junction assembly and barrier function.
MA5-11800 was used in immunohistochemistry and western blot to study the role of CD44 in tight-junction assembly and barrier function
|Kirschner N,Haftek M,Niessen CM,Behne MJ,Furuse M,Moll I,Brandner JM||The Journal of investigative dermatology (131:932)||2011|
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
MA5-11800 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium
|Qi H,Zheng X,Yuan X,Pflugfelder SC,Li DQ||Journal of cellular physiology (225:180)||2010|
Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.
MA5-11800 was used in immunohistochemistry to examine the epithelial differentiation of human embryonic stem cells
|Metallo CM,Azarin SM,Moses LE,Ji L,de Pablo JJ,Palecek SP||Tissue engineering. Part A (16:213)||2010|
Gene transfer in human skin with different pseudotyped HIV-based vectors.
MA5-11800 was used in immunohistochemistry to investigate the efficiency of dermal gene transfer with different pseudotyped HIV-based vectors
|Hachiya A,Sriwiriyanont P,Patel A,Saito N,Ohuchi A,Kitahara T,Takema Y,Tsuboi R,Boissy RE,Visscher MO,Wilson JM,James WM,Kobinger GP||Gene therapy (14:648)||2007|
Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite.
MA5-11800 was used in immunohistochemistry to study the terminal differentiation of umbilical cord epithelial cells
|Sanmano B,Mizoguchi M,Suga Y,Ikeda S,Ogawa H||Journal of dermatological science (37:29)||2005|
Characterization of putative stem cell phenotype in human limbal epithelia.
MA5-11800 was used in immunohistochemistry to evaluate the stem cell molecular markers in human limbal epithelia
|Chen Z,de Paiva CS,Luo L,Kretzer FL,Pflugfelder SC,Li DQ||Stem cells (Dayton, Ohio) (22:355)||2004|
Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.
MA5-11800 was used in immunohistochemistry to study the regulation of epithelial cell differentiation and human thymic medulla Hassall body formation by corticosteroids
|Hale LP,Markert ML||Journal of immunology (Baltimore, Md. : 1950) (172:617)||2004|
Organotypic keratinocyte coculture using normal human serum: an immunomorphological study at light and electron microscopic levels.
MA5-11800 was used in immunohistochemistry to develop an organotypic keratinocyte coculture system with potential for use in autologous skin grafting
|Hinterhuber G,Marquardt Y,Diem E,Rappersberger K,Wolff K,Foedinger D||Experimental dermatology (11:413)||2002|
Bacterial fimbriae and their peptides activate human gingival epithelial cells through Toll-like receptor 2.
MA5-11800 was used in immunohistochemistry to study the role of Toll-like recpeptor-2 in the activation of human gingival epithelial cells by bacterial fimbriae
|Asai Y,Ohyama Y,Gen K,Ogawa T||Infection and immunity (69:7387)||2001|
Growth and differentiation of HaCaT keratinocytes.
MA5-11800 was used in western blot to discuss how changes in calcium levels control differentiation of HaCaT.
|Wilson VG||Methods in molecular biology (Clifton, N.J.) (1195:33)||2014|
Id-1 induces cell invasiveness in immortalized epithelial cells by regulating cadherin switching and Rho GTPases.
MA5-11800 was used in western blot to study the mechanism by which Id-1 induces cell invasiveness in immortalized epithelial cells
|Cheung PY,Yip YL,Tsao SW,Ching YP,Cheung AL||Journal of cellular biochemistry (112:157)||2011|
Methods to promote Notch signaling at the biomaterial interface and evaluation in a rafted organ culture model.
MA5-11800 was used in western blot to evaluate biomaterials suitable for promoting and altering Notch signaling pathway
|Beckstead BL,Tung JC,Liang KJ,Tavakkol Z,Usui ML,Olerud JE,Giachelli CM||Journal of biomedical materials research. Part A (91:436)||2009|
Differential gene expression in primary human skin keratinocytes and fibroblasts in response to ionizing radiation.
MA5-11800 was used in western blot to study the effect of exposure to ionizing radiation on gene expression in human skin keratinocytes and fibroblasts
|Warters RL,Packard AT,Kramer GF,Gaffney DK,Moos PJ||Radiation research (172:82)||2009|
Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation.
MA5-11800 was used in western blot to devise a method for regulating stem cell differentiation using cell-surface ligands bound on a biomaterial surface
|Beckstead BL,Santosa DM,Giachelli CM||Journal of biomedical materials research. Part A (79:94)||2006|
Long-term maintenance of human keratinocytes in vitro.
MA5-11800 was used in western blot to study the long-term maintenance of human keratinocytes in vitro
|Hasskarl J,Velupillai P,Piboonniyom SO,Grace M,Münger K||The Journal of investigative dermatology (124:475)||2005|
Enhanced redundancy in Akt and mitogen-activated protein kinase-induced survival of malignant versus normal prostate epithelial cells.
MA5-11800 was used in western blot to study Akt and MAP kinase-induced survival of malignant versus normal prostate epithelial cells
|Uzgare AR,Isaacs JT||Cancer research (64:6190)||2004|
Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.
MA5-11800 was used in western blot to study the role of the small GTP-binding protein Rap1 in human keratinocyte proliferation
|D'Silva NJ,Mitra RS,Zhang Z,Kurnit DM,Babcock CR,Polverini PJ,Carey TE||Journal of cellular physiology (196:532)||2003|
Microtubule disruption in keratinocytes induces cell-cell adhesion through activation of endogenous E-cadherin.
MA5-11800 was used in western blot to study the involvement of E-cadherin in keratinocyte cell-cell adhesion
|Kee SH,Steinert PM||Molecular biology of the cell (12:1983)||2001|
Low-temperature electrospun silk scaffold for in vitro mucosal modeling.
MA5-11800 was used in immunocytochemistry to evaluate the application of cryogenic electrospun silk scaffold in an in vitro mucosal model
|Bulysheva AA,Bowlin GL,Klingelhutz AJ,Yeudall WA||Journal of biomedical materials research. Part A (100:757)||2012|
Microenvironmental reprogramming of thymic epithelial cells to skin multipotent stem cells.
MA5-11800 was used in immunocytochemistry to investigate the ability of thymic epithelial cells to form skin
|Bonfanti P,Claudinot S,Amici AW,Farley A,Blackburn CC,Barrandon Y||Nature (466:978)||2010|
Isolation and characterization of putative epidermal stem cells derived from Cashmere goat fetus.
MA5-11800 was used in immunocytochemistry to characterize the putative epidermal stem cells from Cashmere goat fetus
|Islam MS,Zhou H||European journal of dermatology : EJD (17:302)||2007|
Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes.
MA5-11800 was used in immunocytochemistry to investigate the role of calprotectin in human gingival keratinocyte differentiation
|Hayashi N,Kido J,Kido R,Wada C,Kataoka M,Shinohara Y,Nagata T||Journal of periodontal research (42:1)||2007|
Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors.
MA5-11800 was used in immunocytochemistry to investigate the possibility of ligand-bearing modified Ad5 vectors to transducing human corneal epithelial progenitor cells
|Chen Z,Mok H,Pflugfelder SC,Li DQ,Barry MA||Experimental eye research (83:798)||2006|
Gap junction protein connexin 43 serves as a negative marker for a stem cell-containing population of human limbal epithelial cells.
MA5-11800 was used in immunocytochemistry to evaluate gap junction protein connexin 43 as a negative marker of the pluripotency of human limbal epithelial cells
|Chen Z,Evans WH,Pflugfelder SC,Li DQ||Stem cells (Dayton, Ohio) (24:1265)||2006|
Organotypic culture and surface plantation using umbilical cord epithelial cells: morphogenesis and expression of differentiation markers mimicking cutaneous epidermis.
MA5-11800 was used in immunocytochemistry to study the ability of umbilical cord epithelial cells to differentiate and form an epidermis-type structure under appropriate conditions
|Mizoguchi M,Suga Y,Sanmano B,Ikeda S,Ogawa H||Journal of dermatological science (35:199)||2004|
Establishment and characterization of immortalized human gingival keratinocyte cell lines.
MA5-11800 was used in flow cytometry to establish and characterize the immortalized human gingival keratinocyte cell lines
|Gröger S,Michel J,Meyle J||Journal of periodontal research (43:604)||2008|