Western blot analysis of mouse Brain Tissue Lysate using PA5-19496, Ionotropic Glutamate receptor 2 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19496 staining of Mouse Cortex Tissue Lysate at a dilution of 1 ug/ml (Lane 2). PA5-19496 staining of Rat Brain Tissue Lysate at a dilution of 1 ug/ml (lane 3). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:300|
|Western Blot (WB)||3-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken and human based on sequence homology.
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate is subject to RNA editing within the second transmembrane domain, which is thought to render the channel impermeable to Caetiology. Alternative splicing, resulting in transcript variants encoding different isoforms, , has been noted for this gene.
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