|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 356 and 686 of JAK1 (Uniprot ID#P23458)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34811 targets JAK1 in WB applications and shows reactivity with Human samples.
The PA5-34811 immunogen is recombinant fragment corresponding to a region within amino acids 356 and 686 of JAK1 (Uniprot ID#P23458).
Janus Kinase 1 (JAK1) is one of a number of related tyrosine kinases involved in cytoplasmic signal transduction. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes.
JAK1 has been shown to be necessary for initiating phosphorylation steps leading to gene induction in the IFN alpha/beta receptor system. In the IL6 response, JAK1 was demonstrated to be essential for sustaining signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.