The PVDF membranes were incubated with mouse anti-JAK3 antibody (Catalog no. AHO1572) at a concentration of 1 µg/ml (lane 1: Jurkat, lane 2: HeLa) for two hours at room temperature. After washing, the membranes were incubated with a goat F(ab')2 anti-mouse IgG HRP conjugated antibody (Catalog no. 65-6420) at a 1:5000 dilution.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human JAK3 N-terminal fragment.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
JAK 3 (also designated L JAK) is a member of the Janus family of protein tyrosine kinases along with JAK 1, JAK 2, or Tyk 2. Subsequent to activation, these kinases function to activate various members of the Stat family of transcription factors by phosphorylation on critical tyrosine regulatory sites. JAK 3 is activated in response to IL-3 and IL-4 in T cells and myeloid cells. JAK 3 activation requires the serine rich membrane proximal region of the IL 2 receptor beta chain, but does not require the acidic domain that is required for association and activation of Src family kinases. Expression of JAK 3 is restricted to NK and T cells.
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