|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant soluble murine JAM-C|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40060 detects JAM-C from mouse and human samples.
MA1-40060 has been successfully used in flow cytometry, ELISA, functional assays, immunohistochemistry (frozen), and immunoprecipitation applications.
The MA1-40060 immunogen is mouse JAM-C.
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The JAM-C/JAM3 protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.