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JMJD1B Antibody (MA5-44640) in ICC/IF
Immunocytochemistry analysis of KDM3B in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in KDM3B Monoclonal antibody (Product # MA5-44640) using a dilution of 1:100 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as t... View More
Product Details
MA5-44640
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1A8
Immunogen
Recombinant protein within human KDM3B aa 251-500.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931100
Target Information
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(9) demethylase 3B; JmjC domain-containing histone demethylation protein 2B; jumonji domain containing 1B; Jumonji domain-containing protein 1B; lysine (K)-specific demethylase 3B; Lysine-specific demethylase 3B; Nuclear protein 5qNCA
Gene Aliases: 5830462I21Rik; 5qNCA; C5orf7; JHDM2B; JMJD1B; KDM3B; KIAA1082; mKIAA1082; NET22
UniProt ID: (Human) Q7LBC6, (Mouse) Q6ZPY7
Entrez Gene ID: (Human) 51780, (Mouse) 277250
antioxidant activity
histone demethylase activity
metal ion binding
dioxygenase activity
sulfonate dioxygenase activity
molecular_function
oxidoreductase activity
2,4-dichlorophenoxyacetate alpha-ketoglutarate dioxygenase activity
procollagen-proline dioxygenase activity
hypophosphite dioxygenase activity
DNA-N1-methyladenine dioxygenase activity
C-19 gibberellin 2-beta-dioxygenase activity
C-20 gibberellin 2-beta-dioxygenase activity
histone modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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