Invitrogen

JMJD2A Recombinant Rabbit Monoclonal Antibody (9A9G6)

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JMJD2A Antibody in Western Blot (WB) — Western Blot analysis of JMJD2A in various cell lines using a JMJD2A Recombinant Monoclonal antibody (Product # MA5-42918) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.

Product Details

MA5-42918

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	9A9G6
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 550-650 of human KDM4A (O75164). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive test controls include: HeLa, NIH/3T3, SH-SY5Y. The target is usually found in the following locations: Nucleus.

Target Information

JMJD2A is a member of the Jumonji domain 2 (JMJD2) family and a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [histone H3]-trimethyl-L-lysine(36) demethylase 4A; [histone H3]-trimethyl-L-lysine(9) demethylase 4A; JmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; Jumonji domain-containing protein 2A; lysine (K)-specific demethylase 4A; Lysine-specific demethylase 4A; tudor domain containing 14A

Gene Aliases: D4Ertd222e; JHDM3A; JMJD2; JMJD2A; KDM4A; KIAA0677; mKIAA0677; TDRD14A

UniProt ID: (Human) O75164, (Mouse) Q8BW72

Entrez Gene ID: (Human) 9682, (Mouse) 230674

Molecular Function: histone modifying enzyme