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Immunofluorescence analysis of JMJD6 in Hela cells or 293T cells using Anti-JMJD6 Recombinant Human Biotinylated Fab (730091). Hela cells or 293T cells were fixed with 3% Formaldehyde and lysed with 1% TritonX-100. Cells were then labeled with Anti-JMJD6 Recombinant Human Biotinylated Fab and detected with Goat-anti-human Alexa Fluor dye F488 (green). FLAG Cells were double labeled with human Fab and detected with Goat-anti-human Alexa Fluor dye F488 (green) as well as with Mouse-anti-FLAG-M2 and detected with Goat-anti-Mouse Alexa Fluor dye F647 (yellow). The DNA in the nucleus was detected with DAPI (Blue). Control means cells were labeled only with secondary antibody Alexa Fluor Dye.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||1-338 of 403 of JMJD6 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||0.5 mg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
Also known as JmjC domain-containing protein 6. This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. It functions in differentiation of multiple tissues during development, and in anti-inflammatory cytokine signaling. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have suggested that the protein may cross-react with a monoclonal antibody that recognizes the phosphatidylserine receptor and does not directly function in the clearance of apoptotic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730091 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gräslund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|
bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; histone arginine demethylase JMJD6; jmjC domain-containing protein 6; jumonji domain containing 6; jumonji domain-containing protein 6; lysyl-hydroxylase JMJD6; peptide-lysine 5-dioxygenase JMJD6; phosphatidylserine receptor; PSR; PTDSR; PTDSR1
JMJD6; KIAA0585; PSR; PTDSR; PTDSR1