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Immunohistochemistry analysis of JNK1/2 showing staining in the cytoplasm and nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a JNK1/2 monoclonal antibody (AHO1362) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human , Mouse , Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant fragment of human JNK1-alpha-1 expressed in E. coli.|
|Storage buffer||PBS, pH 7.2, with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
p54a, JNK2BETA, JNK-55, JNK1A2, PRKM8, PRKM9, JNK2ALPHA, SAPK1a, SAPK1c, AI849689, JNK-46, SAPK1, p54aSAPK, JNK, AI851083, Prkm8, Prkm9, JNK2B, JNK2A, JNK1, JNK21B1/2, JNK2, SAPK
JUN N-terminal kinase, MAP kinase 8, c-Jun N-terminal kinase 1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, stress-activated protein kinase 1, stress-activated protein kinase 1c, Jun kinase, MAP kinase 9, MAPK 9, c-Jun N-terminal kinase 2, c-Jun kinase 2, mitogen-activated protein kinase 9, stress-activated protein kinase 1a, stress-activated protein kinase JNK2, SAPK-alpha, p54-alpha, stress activated protein kinase alpha II, JNK/SAPK alpha, mitogen activated protein kinase 9, protein kinase, mitogen-activated 9, JNK1 beta1 protein kinase, MAPK 8, mitogen activated protein kinase 8, protein kinase mitogen-activated 8, stress-activated protein kinase JNK1, MAPK8, MAPK9, JNK, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, Mitogen-activated protein kinase 9, SAPK1a, JNK2, PRKM9, SAPK1A