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FIGURE: 1 / 10
JunD Antibody (710701) in ICC/IF
Immunofluorescent analysis of JunD was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JunD Recombinant Rabbit Superclonal™ Antibody (Product # 710701) at 5 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 mi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
710701
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
2HCLC
Immunogen
Peptide, multiple (2-16, 132-149, 299-313)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with monkey, sheep, mouse, rat, zebra fish and bovine based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
JunD is a transcription factor which is coded by JunD gene. JunD transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family of protein kinase. Jun proteins are capable of forming dimers with Fos, ATF and CREB family transcription factors to form the AP-1 complex that differentially regulates a variety of target genes involved in cellular growth, proliferation, differentiation and apoptosis. Also, JunD plays an important role in metabolism via modulation of IGF-1 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activator protein 1; AP1; JUN D; jun D proto-oncogene; Oncogene Jun-D; transcription factor AP-1 subunit JunD; transcription factor jun-D; unnamed protein product
Gene Aliases: AP-1
UniProt ID: (Human) P17535
Entrez Gene ID: (Human) 3727
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
circadian rhythm
response to cold
response to water deprivation
response to light stimulus
response to mechanical stimulus
negative regulation of cardiac muscle hypertrophy
response to lipopolysaccharide
positive regulation of peroxisome proliferator activated receptor signaling pathway
regulation of cell proliferation
positive regulation of macrophage activation
response to peptide hormone
positive regulation of transcription from RNA polymerase II promoter
response to mercury ion
response to steroid hormone
epithelial cell proliferation
negative regulation of epithelial cell proliferation
regulation of cell cycle
cellular response to fatty acid
cellular response to hypoxia
response to forskolin
cellular response to polyamine macromolecule
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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