|Tested species reactivity||Dog, Guinea pig, Mouse, Rabbit, Rat|
|Published species reactivity||Rabbit, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rabbit skeletal muscle triads.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
MA3-933 detects the junctional sarcoplasmic reticulum (SR) protein from rabbit and guinea pig; moderately in canine and rat; and weakly in mouse.
MA3-933 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~90 kDa protein representing junctional SR in rabbit skeletal muscle extracts. Immunofluorescence staining of junctional sarcoplasmic reticulum with MA3-933 results in an increase in intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle, and does not cross-react with cardiac muscle.
The MA3-933 antigen is rabbit skeletal muscle triads.
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR and quote;s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR and quote;s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR and quote;s and RyR and quote;s.
Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Microarray profiling of skeletal muscle sarcoplasmic reticulum proteins.
MA3-933 was used in western blot to investigate the skeletal muscle sarcoplasmic reticulum proteins through microarray analyses
|Schulz JS,Palmer N,Steckelberg J,Jones SJ,Zeece MG||Biochimica et biophysica acta (1764:1429)||2006|
Increased expression of the nicotinic acetylcholine receptor in stimulated muscle.
MA3-933 was used in western blot to study the effect of chronic low frequency stimulation on nicotinic acetylcholine receptor expression in skeletal muscle
|O'Reilly C,Pette D,Ohlendieck K||Biochemical and biophysical research communications (300:585)||2003|
Identification of a novel 45 kDa protein (JP-45) from rabbit sarcoplasmic-reticulum junctional-face membrane.
MA3-933 was used in western blot to characterize a novel 45kDa protein isolated from rabbit skeletal muscle sarcoplasmic reticulum junctional face membranes
|Zorzato F,Anderson AA,Ohlendieck K,Froemming G,Guerrini R,Treves S||The Biochemical journal (351 Pt 2:537)||2000|
Comparative analysis of the isoform expression pattern of Ca(2+)-regulatory membrane proteins in fast-twitch, slow-twitch, cardiac, neonatal and chronic low-frequency stimulated muscle fibers.
MA3-933 was used in western blot to study the expression of Ca(2+) regulatory membrane protein isoforms in skeletal, cardiac and neonatal muscle fibres
|Froemming GR,Murray BE,Harmon S,Pette D,Ohlendieck K||Biochimica et biophysica acta (1466:151)||2000|