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Alomone Labs, LTD
KCNK16 (TALK-1) (extracellular) Polyclonal Antibody
FIGURE: 1 / 2
KCNK16 (TALK-1) (extracellular) Antibody (APC-170-25UL) in WB
Western blot analysisof human PANC-1 pancreas ductal adenocarcinoma cell line lysate: - 1. Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170), (1:600). 2. Anti-KCNK16 (TALK-1) (extracellular) Antibody , preincubated with KCNK16/TALK-1 (extracellular) Blocking Peptide (#BLP-PC170).
Product Details
APC-170-25UL
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16, 1st extracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human Beta-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3). The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction. TALK-1, two-pore domain K+ (K2p 16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on Beta-cell CA2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2P domain potassium channel Talk-1; pancreatic potassium channel Talk-1; Potassium channel subfamily K member 16; potassium channel, subfamily K, member 16; potassium channel, two pore domain subfamily K, member 16; TALK-1; TWIK-related alkaline pH-activated K(+) channel 1
Gene Aliases: 4731413G05Rik; K2p16.1; KCNK16; TALK-1; TALK1
UniProt ID: (Human) Q96T55
Entrez Gene ID: (Human) 83795, (Mouse) 74571, (Rat) 688996
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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