Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
1:300 | - |
Immunocytochemistry (ICC/IF) |
1:300 | - |
Miscellaneous PubMed (Misc) |
- | View 11 publications 11 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Not Applicable |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
UMAB107 |
Immunogen |
Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 produced in E.coli. |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
0.5-1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS with 1% BSA, 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2
Gene Aliases: KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013
Entrez Gene ID: (Human) 4288
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